Experimental Sleep Studies, Universidad Autonoma de Madrid

Anatomía, Histología y Neurociencia

Contact:
Isabel Trinidad DE ANDRES
Position:
Full Professor
Address:
Arzobispo Morcillo 4, 28029
City:
Madrid
Country:
Spain
Phone:
34 91 4975337
Fax:
34 91 4975338
Contact E-mail:
Research Domain
Keywords
Current Announcements

Neurobiology of Sleep and its Disorders. (Posgraduate course within the Master in Neuroscience. Universidad Autonoma of Madrid). May 2012


Laboratory description

1. Neuroanatomical, neurophysiological and neurochemical sleep mechanisms.
2. Acute and chronic effects of opiates on sleep and EEG


Technical Capabilities and areas of expertise

Sterotaxic surgery in experimental animals
Polygraphic recordings in freely moving cats
Neuronal unit activity in anesthetized rats
Intracerebral microinjections
Neuronal tracers. Histochemistry and Immunochemistry
Light and electron microscope studies


Additional Descriptors, Keywords

Acetylcholine, carbachol, morphine, GABA, hypocretin/orexin, pontine tegmentum, basal forebrain


Selected Publications in the 5 past years:

-De Andres, I., M. Garzón and F. Reinoso- Suárez . Functional anatomy of non-REM sleep. Front Neurol. 2:70. 2011

-Villablanca JR and I. de Andrés. Understanding REM sleep clues from brain lesion studies. In: Rapid Eye Movement Sleep: Mechanism of Regulation and Dysregulation. Eds. BN Mallick, SR Pandi-Perumal, R McCarley and A Morisson. Cambrige University Press . UK. pp. 89-98. 2011.

-Reinoso-Suárez F., I. de Andrés and M. Garzón.Functional Anatomy of the Sleep-Wakefulness Cycle: Wakefulness.Adv Anat Embryol Cell Biol; 208:1-128. 2011

-Del Cid-Pellitero E. and M. Garzón. Hypocretin1/OrexinA axon targeting of Laterodorsal Tegmental Nucleus neurons projecting to the rat medial prefrontal cortex Cereb Cortex. 12:2762-73. 2011

-Del Cid-Pellitero E, M. Garzón. Hypocretin1/OrexinA-containing axons innervate locus coeruleus neurons that project to the Rat medial prefrontal cortex. Implication in the sleep-wakefulness cycle and cortical activation. Synapse 65:843-57. 2011

-Reinoso-Suárez F., C. De la Roza, M.L. Rodrigo-Angulo, I. De Andrés, A. Núñez and M. Garzón. GABAergic mechanisms in the ventral oral pontine tegmentum -the REM sleep induction site- in the modulation of sleep-wake states.In: GABA and Sleep – Molecular, Functional & Clinical Aspects. Ed. J.M. Monti, S.R. Pandi-Perumal and H. Möhler. Birkhauser-Verlag, Basel, pp. 233-252. 2010.

-Del Cid-Pellitero E., M. Garzón. Medial prefrontal cortex receives input from dorsal raphe nucleus neurons targeted by Hypocretin1/OrexinA-containing axons. Neuroscience 172:30-43. 2010.

-Núñez A, ML Rodrigo-Angulo, I. de Andrés and M. Garzón. Hypocretin/orexin neuropeptides: Participation in the control of sleep-wakefulness cycle and energy homeostasis. Current Neuropharmacol., 7: 50-59 2009.

-de la Roza C, F. Reinoso-Suárez. Ultrastructural characterization of relationship between serotonergic and GABAergic structures in the ventral part of the oral pontine reticular nucleus. Neuroscience. 15;164:1180-90. 2009

-Moreno-Balandrán ML, M. Garzón, C. Bódalo, F. Reinoso-Suárez and I. de Andrés. Sleep-wakefulness effects after microinjections of hypocretin 1 (orexin A) in cholinoceptive areas of the cat oral pontine tegmentum. Eur. J. Neurosci. 28: 331–341. 2008

-Rodrigo-Angulo ML, S. Heredero, E. Rodríguez-Veiga, F. Reinoso-Suárez. GABAergic and non-GABAergic thalamic, hypothalamic and basal forebrain projections to the ventral oral pontine reticular nucleus: their implication in REM sleep modulation.
Brain Res. 1210:116-25. 2008

-Núñez A, ME Moreno-Balandrán, ML Rodrigo-Angulo, M. Garzón and I. de Andrés. Relationship between the perifornical hypothalamic area and the oral pontine reticular nucleus in the rat. Possible implication of the hypocretinergic projection in the control of REM sleep. Eur. J. Neurosci. 24:2834-2842. 2006

-de la Roza C, F. Reinoso-Suárez. GABAergic structures in the ventral part of the oral pontine reticular nucleus: An ultrastructural immunogold analysis. Neuroscience. 142:1183-93. 2006


Key Publications of the Laboratory:

Reinoso-Suárez F. Die Auswirkungen der Ausschaltung eines Nucleus ruber auf die Hirnrinde (Electroencephalographische Untersuchungen an der Katze). Dtsch.Z.Nervenheilk. 172: 201-219. 1954.
Camacho A., F. Reinoso-Suárez. Activating and synchronizing centers in cat brain. Science. 146: 268-270. 1964.
Obrador S., F. Reinoso-Suárez, J. Carbonell, A. Córdoba, E. Martínez-Moreno, V. Navarro, H. Oliva, JC. Oliveros. Comatose state maintained during eight years following a vascular ponto mesencephalic lesion. Electroenceph. Clin. Neurophysiol. 38: 21-26. 1975.
Llamas A., F. Reinoso-Suárez, E. Martínez-Moreno. Projections to the gyrus proreus from the brain stem tegmentum (locus coeruleus, raphe nuclei) in the cat, demonstrated by retrorade transport of horseradish peroxidase. Brain Res. 89: 331-336. 1975.
De Andrés I. and F. Reinoso-Suárez. Cerebellar participation in the regulation of sleep-wakefulness cycle through the superior cerebellar peduncle. Arch. ital. Biol. ll7:l40-l63. l979.
Cunchillos J.D. and I. de Andrés. Participation of the cerebellum in the regulation of the sleep- wakefulness cycle. Results in cerebellectomized cats. Electroenceph. Clin. Neurophysiol. 53:549-558. l982.
Caballero A. and I. de Andrés. Unilateral lesions in the Locus coeruleus area enhance paradoxical sleep. Electroenceph. Clin. Neurophysiol. 64:339-346. 1986.
Corpas I. and I. de Andrés. Morphine effects in brainstem transected cats : I. EEG and sleep-wakefulness in the isolated forebrain. Beh. Brain Res. 44: 11-19. 1991
De Andrés I. and I. Corpas. Morphine effects in brainstem transected cats : II. Sleep and behavior of the decerebrate cat. Beh. Brain Res. 44: 21-26. 1991.
Nuñez A., I. de Andrés and E. García-Austt. Relationships of the nucleus reticularis pontis oralis neuronal activity with sensory and carbachol evoked hippocampal theta rhytm. Exp.Brain Res. 87: 303-308. 1991.
Arpa J. and I. de Andrés. Re-examination of the effects of raphe lesions on the sleep-wakefulness cycle states in cats. J. Sleep Res. 2:96-102. 1993.
Reinoso-Suárez F., I. de Andrés, ML. Rodrigo-Angulo and E. Rodriguez-Veiga. Location and anatomical connections of a paradoxical sleep induction site in the brainstem of the cat. Eur. J. Neurosci. 6 : 1829-1836. 1994
Reinoso-Barbero F. and I. de Andrés. Effects of opioid microinjections in the nucleus of the solitary tract on the sleep-wakefulness cycle states in cats. Anesthesiology. 82: 144-152. 1995.
Garzón, M., I. de Andrés, and F. Reinoso-Suárez. Neocortical and hippocampal electrical activities are similar in spontaneous and cholinergic-induced REM sleep. Brain Res. 766: 266-270. 1997.
Núñez A., C.de la Roza, ML. Rodrigo-Angulo, W. Buño and F.Reinoso-Suárez. Electrophysiological properties and cholinergic responses of rat ventral oral pontine reticular neurons in vitro. Brain Res. 754: 1-11. 1997.
Garzón M., I. de Andrés, and F. Reinoso-Suárez. Sleep patterns after carbachol delivery in the ventral oral pontine tegmentum of the cat. Neuroscience 83:1137-1144. 1998.
Núñez A., W.Buño, F.Reinoso-Suárez. Neurotransmitter actions on oral pontine tegmental neurons of the rat: an in vitro study. Brain Res. 804: 144-148, 1998.
Rodrigo-Angulo ML., E. Rodríguez-Veiga and F. Reinoso-Suárez . Serotonergic connections to the ventral oral pontine tegmentum. Implication in paradoxical sleep modulation. J. Comp. Neurol. 418: 93-105. 2000.
de la Roza C., F.Reinoso-Suárez. Ultrastructure and synaptic organization of axon terminals of the ventral part of the oral pontine reticular nucleus. J. Comp. Neurol. 418:93-105, 2000
Reinoso-Suárez F., I. de Andrés, ML. Rodrigo-Angulo, and M. Garzón. Brain structures and mechanisms involved in the generation of Rem sleep. Sleep Med. Rev. 5: 63-77. 2001.
Villablanca JR., I. de Andrés and Ch E Olmstead. Sleeping and waking develop independently in the isolated forebrain and decerebrate cat after a mesencephalic transection early postnatally. Neuroscience 106: 717-731. 2001.
Núñez A., ML. Rodrigo, I. de Andrés and F. Reinoso- Suárez. Firing activity and postsynaptic propierties of morphologically identified neurons of the ventral oral pontine reticular nucleus. Neuroscience. 115: 1165-1175. 2002.
De Andrés I., M. Garzón and J. Villablanca. The disconnected brain stem does not support rapid eye movement sleep rebound following selective deprivation. Sleep. 26: 419-435; 2003.
De Andrés I., M. Garzón and JR. Villablanca. The brain stem but not the forebrain independently supports basic morphine´s tolerance and withdrawal effects. A study in cats with midbrain transection Beh. Brain Res. 148:133-144. 2004.
Rodrigo-Angulo ML., E. Rodriguez-Veiga and F. Reinoso-Suarez . A quantitative study of the brainstem cholinergic projections to the ventral part of the oral pontine reticular nucleus (REM sleep induction site) in the cat. Exp Brain Res. 160:334-343, 2005.


Current/Completed Grants (past 5 years):

Bases Morfofuncionales de la modulación hipotalámica en la regulación pontina del sueño REM (FPU 2009-06991. Spanish Ministry of Science and Innovation)
2009-2012

-Estudio interdisciplinar (neuroquímico, conectivo, poligráfico, electrofisiológico y ultraestructural) de la regulación orexinérgica de los estados de vigilia y sueño: Interacciones a nivel del tegmento pontino y el prosencéfalo basal. (BFI 2006-07430. Spanish Ministry of Science and Tecnology).
2006 -2009


Other laboratory Members

Title:  PhD MD

Name:  Miguel Garzon

Position:  Full professor

Email:  miguel.garzon@uam.es

Title:  PhD MD

Name:  Fernando Reinoso

Position:  Emeritus Professor

Email:  fernando.reinoso@uam.es

Title:  PhD

Name:  Angel Nuñez

Position:  Full professor

Email:  angel.nunez@uam.es

Name:  Margarita Lucia Rodrigo-Angulo

Position:  Assistant professor

Email:  marga.rodrigo@uam.es

Name:  Carmen De la Roza

Position:  Assistant professor

Email:  carmen.roza@uam.es

Title:  PhD

Name:  Omar Robayo

Position:  Postdoctoral student

Email:  omar.robayo@uam.es

Name:  Cristina Bodalo

Position:  Doctoral Student

Email:  cristina.bodalo@uam.es

Title:  MSc

Name:  Marta Carus

Position:  Doctoral student

Email:  marta.carus@uam.es

Title:  MSc

Name:  Luis Asensio

Position:  Pregraduate student

Email:  luis.asensio@estudiante.uam.es

Title:  BSc

Name:  Iago Rego

Position:  Pregraduate student

Email:  iago.rego@estudiante.uam.es


Collaborations

Title:  PhD MD

Name:  Jaime R Villablanca

Position:  Distinguished Professor Emeritus

Institution:  Mental Retardation Research Center, Brain Research Institute, University of California, Los Angeles

Name:  Virginia M Pickel

Position:  Full Professor

Institution:  Weill Medical College of Cornell University New York

Title:  PhD

Name:  Julio Rodriguez

Position:  Research Professor

Institution:  Universidad del País Vasco

Title:  PhD

Name:  Elisa Mengual

Position:  Research Professor

Institution:  Universidad de Navarra

Title:  PhD

Name:  Ester Luqin

Position:  Research fellow

Institution:  Universidad de Navarra

Title:  PhD MD

Name:  Javier Arpa

Position:  Neurology

Institution:  Hospital Universitario La PAZ

© 2019 European Sleep Research Society. All rights reserved.
X