Sleep Science Friday: Pain and Sleep


Painting by Anne Durrant-Cardis

Article Review – Cardis et al. (2021). Cortico-autonomic local arousals and heightened somatosensory arousability during NREMS of mice in neuropathic pain. Elife.

17 September 2021

Text by Anita Lüthi and Isabelle Decosterd






We all have experienced pain, and we also know the relief when pain finally fades. However, not everyone who is in pain is that fortunate. Disease or injuries to the nervous system, can lead to chronic neuropathic pain that persists and is unfortunately often intractable. Moreover, as the body hurts for months or years, a negative sensory and emotional state overwhelms the patient – including during sleep. Not surprisingly, therefore, chronic pain and sleep disturbances often coexist, with patients showing increased latency to fall asleep, poorer sleep efficiency and increased tendency to wake up during the night. The fatigue that arises on top of sensory and emotional discomfort creates a malicious mix that aggravates a condition that is already difficult to manage.


What brought us to start a project on pain and sleep

We reasoned that if we could capture the moments when pain intrudes into sleep and disrupts it, we could advance strategies to anticipate and combat pain-related sleep disruptions. For this idea, it is important to know that even the healthiest of us sleepers wakes up spontaneously during the night, although briefly so and variably engaging the brain, the heart and/or limb muscles. If we better understand the rules by which such spontaneous wake-ups take place, we should be able to estimate sleep’s vulnerability to pathological sensory activity, such as the one experienced in chronic pain.


Our findings on sleep in an experimental mouse model of neuropathic pain

Rodent experimental models of chronic pain have allowed break-through studies on chronic pain mechanisms, yet studies on sleep in these models are scarce. By inspecting when the sleeping brain of a healthy mouse becomes vulnerable to spontaneous brief awakenings, we have identified a time interval of close to a minute that allowed us to anticipate the most fragile moments of non-rapid-eye-movement (NREM) sleep across the light-dark cycle. We hence used this raster to inspect NREM sleep in an experimental model of neuropathic pain in mice, caused by a nerve injury of the sciatic nerve. These mice overall seemed to sleep for normal durations at normal periods of the light-dark cycle. However, when we inspected their brain activity at the most vulnerable moments, we found that the cortical brain area treating sensory information from the injured hindlimb showed wake-like brain activity more frequently in brain areas concerned with the sensory processing of pain (Figure 1, top). Moreover, at these moments, their heart rate was also higher. These mice thus showed a novel type of local cortical arousal combined with autonomic heart activity. These physiological signs of local arousal are reminiscent of observations in insomnia patients, in which arousal-like changes in oscillatory electrical activity appear over brain areas involved in sensorimotor activity. Additionally, mice with spared nerve injury also woke up more frequently to external fine vibrations, indicating a heightened vulnerability to external disturbances (Figure 1, bottom).


What we conclude

The essence of the sleep disturbances in this neuropathic pain model thus relies in an abnormal arousability, in both its spontaneous and evoked forms. The study thus demonstrates that rodent pain models provide insight into the human condition, in which more frequent awakenings are amongst the most consistent effects reported. The identification of a novel type of arousal in mouse in fact revives the need for an accurate assessment of arousability in humans in the context of a variety of sleep disorders and search for new therapy approaches in order to improve the quality of life of people suffering from neuropathic pain.


Figure legend

Graphic illustration of abnormal arousability from non-rapid-eye-movement sleep (NREMS) in the spared-nerve-injury mouse model of neuropathic pain. Top, Modification of spontaneous arousability in NREMS. Red line show the infraslow variations in spontaneous arousability (measured in the sigma frequency band, 10-15 Hz) in healthy mice that we describe in this study, labeling in particular the fragile sleep period. We also show that the experimental model mice show more frequent arousals that are a) local, because they concern the pain-afflicted cortical area, and b) autonomic, because they are accompanied by an increased heart rate. Bottom, Modification of evoked arousability. Mice in NREMS were exposed to mild mechanovibrational stimuli during the fragile sleep period indicated on the top, tuned to cause wake-ups in about half of the trials. Mice in neuropathic pain conditions showed more frequent wake-ups compared to sham animals.

ESRS Announcements

Sleep Europe 2022 Call for Symposia 

We’d like to invite all members to contribute to the program by proposing a symposium. Submissions are now being accepted until 27 September 2021. Find out more on the criteria and procedures here.  


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Recent publications from ESRS members: 

  1. Cordani et al. (2021). Sleep disturbances in craniopharyngioma: a challenging diagnosis. J Neurol.
  2. Jaramillo et al. (2021). Thalamic influence on slow wave slope renormalization during sleep. Ann Neurol.
  3. Leppänen et al. (2021). Beyond the apnea-hypopnea index: alternative diagnostic parameters and machine learning solutions for estimation of sleep apnea severity. Sleep.
  4. Kovalzon & Lyamin (2021). In memory of Lev Mukhametov. J Sleep Res.
  5. Massie et al. (2021). Phenotyping REM OSA by means of peripheral arterial tone-based home sleep apnea testing and polysomnography: A critical assessment of the sensitivity and specificity of both methods. J Sleep Res.
  6. Bartolucci et al. (2021). Obstructive sleep apneas naturally occur in mice during rem sleep and are highly prevalent in a mouse model of down syndrome. Neurobiol Dis.
  7. Tarokh & Vyazovskiy (2021). Too sleepy for school: is sleep in teenagers homeostatically regulated under chronic sleep restriction? Sleep.
  8. Salfi et al. (2021). Changes of evening exposure to electronic devices during the COVID-19 lockdown affect the time course of sleep disturbances. Sleep.
  9. Skorucak et al. (2021). Homeostatic response to sleep restriction in adolescents. Sleep.
  10. Simor et al. (2021). Cortical monitoring of cardiac activity during rapid eye movement sleep: the heartbeat evoked potential in phasic and tonic rapid-eye-movement microstates. Sleep.

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